RESUMO
Toxocariasis is a widespread helminth infection of dogs and cats, caused by Toxocara canis and Toxocara cati larvae, respectively. Toxocara spp. can cause zoonotic infections in humans by invading tissues and organs causing pathology. Toxocara spp. larvae release excretory-secretory molecules (TES) into the body of their host that are fundamental to the host-parasite interaction and could be used as targets for novel diagnostics and vaccines. In the present study, we identified 646 T. canis proteins from TES and larval extract using 1D-SDS PAGE followed by mass spectrometry. A wide range of proteins was identified that may play a role both in the induction of the host immune response and host pathology, and in parasite metabolism and survival. Among these proteins there are potential candidates for novel diagnostics and vaccines for dogs and cats toxocariases.
Assuntos
Proteínas de Helminto/análise , Proteínas de Helminto/metabolismo , Larva/química , Proteômica , Toxocara canis/química , Animais , Antígenos de Helmintos/imunologia , Bases de Dados Genéticas , Cães , Eletroforese em Gel de Poliacrilamida , Feminino , Proteínas de Helminto/genética , Proteínas de Helminto/isolamento & purificação , Humanos , Toxocara canis/patogenicidade , Toxocaríase/parasitologia , Zoonoses/parasitologia , Zoonoses/transmissãoRESUMO
Vascular endothelial growth factors (VEGFs) are among the most important angiogenic proteins found on vertebrates. In the last years, some reports of the occurrence of such proteins in snake venoms are rising the importance of this family of proteins as toxins, since they appear to be involved in many features of Viperidae envenoming, such as hypotension and venom spread through increase in vascular permeability. Here we describe the occurrence of snake venom VEGF in Bothrops erythromelas, a clinical important snake from Northeast of Brazil, through immunodetection and cloning of its cDNA and briefly provide an overview comparison of all recent described svVEGF sequences.
